Home Print this page Email this page Small font sizeDefault font sizeIncrease font size
Users Online: 452
Home | About us | Editorial board | Search | Ahead of print | Current issue | Archives | Submit article | Instructions | Subscribe | Contacts | Login 
Year : 2013  |  Volume : 3  |  Issue : 1  |  Page : 44-52

Immunodiagnosis of fascioliasis using sandwich enzyme-linked immunosorbent assay for detection of Fasciola gigantica paramyosin antigen

1 Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt
2 Department of Parasitology, Theodore Bilharz Research Institute, Giza, Egypt

Correspondence Address:
Hany Mohamed Adel Abou-Elhakam
Villa 330, First Touristic Village, 6th of October City, Cairo
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/2229-5070.113907

Rights and Permissions

Background: Many immunological techniques have been developed over years using the different Fasciola antigens for diagnosis of parasitic infection and to replace the parasitological techniques, which are time consuming and usually lack sensitivity and reproducibility. Materials and Methods: In this study, Fasciola gigantica paramyosin (Pmy) antigen was early detected in cattle sera using sandwich enzyme-linked immunosorbent assay (ELISA), to evaluate the Pmy antigen performance in diagnosis. This work was conducted on 135 cattle blood samples, which were classified according to parasitological investigation into, healthy control (30), fascioliasis (75), and other parasites (30) groups. Results: The sensitivity of Sandwich ELISA was 97.33%, and the specificity was 95%, in comparison with parasitological examination, which recorded 66.66% sensitivity and 100% specificity, respectively. Conclusions: It was clear that the native F. gigantica Pmy is considered as a powerful antigen in early immunodiagnosis of fascioliasis, using a highly sensitive and specific sandwich ELISA technique.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded196    
    Comments [Add]    
    Cited by others 4    

Recommend this journal