Home Print this page Email this page Small font sizeDefault font sizeIncrease font size
Users Online: 136
Home | About us | Editorial board | Search | Ahead of print | Current issue | Archives | Submit article | Instructions | Subscribe | Contacts | Login 
Year : 2022  |  Volume : 12  |  Issue : 2  |  Page : 87-93

Evaluation of microscopy and PCR for detection of Dientamoeba fragilis

1 Department of Parasitology, Medical Research Institute, University of Alexandria, Alexandria, Egypt
2 Department of Zoology, Faculty of Science, University of Alexandria, Alexandria, Egypt

Correspondence Address:
Amel Youssef Shehab
Department of Parasitology, Medical Research Institute, 165 El Horreya Avenue, El Hadara, Alexandria
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/tp.tp_93_21

Rights and Permissions

Introduction: Dientamoeba fragilis (D. fragilis) diagnosis is an intestinal protozoan parasite globally found in rural and urban areas and is attracting a growing interest. Its prevalence in stool varies from 0.2% to more than 19% depending upon the population studied. Materials and Methods: This study was based on the examination of 100 stool samples of randomly referred cases in a rural area in Motobus district, Kafr El-Sheikh governorate, Egypt. Our aim was to investigate the presence of D. fragilis in stool of the examined individuals using conventional polymerase chain reaction (PCR) compared to wet mount and trichrome stain with confirmation of infection by transmission electron microscopy. Results: D. fragilis was detected in 13/100 of the stool samples examined using wet mount smears, while trichrome stain detected 17/100. Conventional PCR diagnosed 41 cases of D. fragilis in the studied group. A very good agreement was found between wet mount and trichrome stain for diagnosing D. fragilis, while there was fair agreement between conventional PCR and both microscopy methods. Transmission electron microscope was performed on pooled positive samples that revealed the internal structures of D. fragilis trophozoite with its characteristic nucleus. Conclusions: PCR technique was superior to microscopy for the detection of D. fragilis. Trichrome stain remains vital for microscopic diagnosis.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded7    
    Comments [Add]    

Recommend this journal